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A long-term stability study (over an 8-month period at 4, −4 and −20°C) of 26 sedative−hypnotics including alprazolam was carried out in six toxicological matrices: ante-mortem blood in potassium oxalate: sodium fluoride (1:10), post-mortem blood in potassium oxalate: sodium fluoride (1:10), post-mortem urine in sodium fluoride, homogenized liver (20 g diluted 1:3 with deionized water), homogenized brain (20 g diluted 1:2 with deionized water), and homogenized gastric contents (1:100 with deionized water)

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The infant intestinal flora is rich in genes for de novo synthesis of folate, while the adult flora is rich in genes related to the metabolism of folate and its reduced form THF (18)